Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification

  • Purification of mRNA with oligo(dT)-functionalized magnetic particles involves a series of magnetic separations for buffer exchange and washing. Magnetic particles interact and agglomerate with each other when a magnetic field is applied, which can result in a decreased total surface area and thus a decreased yield of mRNA. In addition, agglomeration may also be caused by mRNA loading on the magnetic particles. Therefore, it is of interest how the individual steps of magnetic separation and subsequent redispersion in the buffers used affect the particle size distribution. The lysis/binding buffer is the most important buffer for the separation of mRNA from the multicomponent suspension of cell lysate. Therefore, monodisperse magnetic particles loaded with mRNA were dispersed in the lysis/binding buffer and in the reference system deionized water, and the particle size distributions were measured. A concentration-dependent agglomeration tendency was observed in deionized water. In contrast, no significant agglomeration was detected in the lysis/binding buffer. With regard to magnetic particle recycling, the influence of different storage and drying processes on particle size distribution was investigated. Agglomeration occurred in all process alternatives. For de-agglomeration, ultrasonic treatment was examined. It represents a suitable method for reproducible restoration of the original particle size distribution.

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Author:Lars Wommer, Winda Soerjawinata, Roland Ulber, Percy Kampeis
URN:urn:nbn:de:hbz:tr5-1107
DOI:https://doi.org/10.1002/elsc.202000112
Parent Title (English):Engineering in Life Sciences
Publisher:Wiley
Document Type:Article (specialist journals)
Language:English
Date of OPUS upload:2022/09/03
Date of first Publication:2021/06/24
Publishing University:Hochschule Trier
Release Date:2022/09/05
Tag:agglomeration; de-agglomeration; high-gradient magnetic separation; mRNA-vaccines; magnetic beads
GND Keyword:Magnetisches Trennverfahren; mRNA-Impfstoff; Agglomerieren
Volume:21
Issue:10
Page Number:15
First Page:558
Last Page:572
Departments:FB Umweltplanung/-technik (UCB)
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International