TY  - JOUR
A1  - Wommer, Lars
A1  - Soerjawinata, Winda
A1  - Ulber, Roland
A1  - Kampeis, Percy
T1  - Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification
T2  - Engineering in Life Sciences
N2  - Purification of mRNA with oligo(dT)-functionalized magnetic particles involves a series of magnetic separations for buffer exchange and washing. Magnetic particles interact and agglomerate with each other when a magnetic field is applied, which can result in a decreased total surface area and thus a decreased yield of mRNA. In addition, agglomeration may also be caused by mRNA loading on the magnetic particles. Therefore, it is of interest how the individual steps of magnetic separation and subsequent redispersion in the buffers used affect the particle size distribution. The lysis/binding buffer is the most important buffer for the separation of mRNA from the multicomponent suspension of cell lysate. Therefore, monodisperse magnetic particles loaded with mRNA were dispersed in the lysis/binding buffer and in the reference system deionized water, and the particle size distributions were measured. A concentration-dependent agglomeration tendency was observed in deionized water. In contrast, no significant agglomeration was detected in the lysis/binding buffer. With regard to magnetic particle recycling, the influence of different storage and drying processes on particle size distribution was investigated. Agglomeration occurred in all process alternatives. For de-agglomeration, ultrasonic treatment was examined. It represents a suitable method for reproducible restoration of the original particle size distribution.
KW  - Magnetisches Trennverfahren
KW  - mRNA-Impfstoff
KW  - Agglomerieren
KW  - agglomeration
KW  - de-agglomeration
KW  - high-gradient magnetic separation
KW  - magnetic beads
KW  - mRNA-vaccines
Y1  - 2021
UR  - https://hst.opus.hbz-nrw.de/frontdoor/index/index/docId/110
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:tr5-1107
VL  - 21
IS  - 10
SP  - 558
EP  - 572
PB  - Wiley
ER  -