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Decoding the cellular network interaction of neurons and glial cells are important in the development of new therapies for diseases of the central nervous system (CNS). Electrophysiological in vivo studies in mice will help to understand the highly complex network. In this paper, the optimization of epidural liquid crystal polymer (LCP) electrodes for different platinum electroplating parameters are presented and compared. Constant current and pulsed current electroplating varied in strength and duration was used to decrease the electrode impedance and to increase the charge storage capacity (CSCc). In best cases, both methods generated similar results with an impedance reduction of about 99%. However, electroplating with pulsed currents was less parameter-dependent than the electroplating with constant current. The use of ultrasound was essential to generate platinum coatings without plating defects. Electrode model parameters extracted from the electrode impedance reflected the increase in surface porosity due to the electroplating processes.
Electrical stimulation is used for example to treat neuronal disorders and depression with deep brain stimulation or transcranial electrical stimulation. Depending on the application, different electrodes are used and thus different electrical characteristics exist, which have to be handled by the stimulator. Without a measuring device the user would have to rely on the stimulator being able to deliver the needed stimulation signal. Therefore, the objective of this paper is to present a method to increase the level of confidence with characterization and modelling of the electrical behavior by using the example of one channel of our stimulation device for experimental use. In several simulation studies with an electrode model with values in a typical range for cortical applications the influence of the load onto the stimulator and the possibility to pre-estimate measuring signals in complex networks are shown.
Understanding and modulating CNS function in physiological as well as pathophysiological contexts remains a significant ambition in research and clinical applications. The investigation of the multifaceted CNS cell types including their interactions and contributions to neural function requires a combination of the state-of-the-art in vivo electrophysiology and imaging techniques. We developed a novel type of liquid crystal polymer (LCP) surface micro-electrode manufactured in three customized designs with up to 16 channels for recording and stimulation of brain activity. All designs include spare central spaces for simultaneous 2P-imaging. Nanoporous platinum-plated contact sites ensure a low impedance and high current transfer. The epidural implantation of the LCP micro-electrodes could be combined with standard cranial window surgery. The epidurally positioned electrodes did not only display long-term biocompatibility, but we also observed an additional stabilization of the underlying CNS tissue. We demonstrate the electrode’s versatility in combination with in vivo 2P-imaging by monitoring anesthesia-awake cycles of transgenic mice with GCaMP3 expression in neurons or astrocytes. Cortical stimulation and simultaneous 2P Ca2+ imaging in neurons or astrocytes highlighted the astrocytes’ integrative character in neuronal activity processing. Furthermore, we confirmed that spontaneous astroglial Ca2+ signals are dampened under anesthesia, while evoked signals in neurons and astrocytes showed stronger dependency on stimulation intensity rather than on various levels of anesthesia. Finally, we show that the electrodes provide recordings of the electrocorticogram (ECoG) with a high signal-to noise ratio and spatial signal differences which help to decipher brain activity states during experimental procedures. Summarizing, the novel LCP surface micro-electrode is a versatile, convenient, and reliable tool to investigate brain function in vivo.
Zur Optimierung von Zulaufsatzkultur-Fermentationen von methylotrophen Organismen wird eine Online-Messmethode vorgestellt, mit der die Methanol-Konzentration im Medium während einer Fermentation durch ein Spülgaspervaporations-Prinzip bestimmt werden kann. Im Gegensatz zu anderen Analysemethoden bietet die Messmethode die Möglichkeit, die Substratkonzentration bei Prozessen mit Methanol als zentralem Substrat über eine Regelung auf einem definierten Wert zu halten. Es werden Schwierigkeiten, aber auch deren Überwindung bei der Adaption der Messmethode auf Fermentationsprozesse dargestellt.
Die vorliegende Ausarbeitung beschäftigt sich mit der Frage, ob weibliche Führungskräfte aufgrund spezieller Führungskompetenzen auf den langfristigen Unternehmenserfolg einzahlen. Besondere Beachtung findet hierbei der Faktor der Emotionalen Intelligenz. Nach wie vor ist der Anteil weiblicher Führungskräfte auf deutschen Managementebenen auffallend gering. Um ein Verständnis für die Hintergründe dieser Thematik zu entwickeln, werden zunächst die Ursachen geschlechtsbedingter Differenzierungen erläutert. Die wesentlichen Erkenntnisse entstammen dem Bereich der Geschlechterforschung. Im Anschluss werden diese im Kontext der Führung beleuchtet. Ein besonderes Augenmerk liegt auf der Frage, ob und inwiefern sich ein potentieller weiblicher Führungsstil von der als männlich geltenden Führung unterscheidet. Im weiteren Verlauf des Artikels wird das Phänomen der Emotionalen Intelligenz im Detail betrachtet. Dieses gilt im Kontext der Führung seit geraumer Zeit als Erfolgsfaktor. Ob und inwiefern diese Annahme tatsächlich Bestand hat, soll daher zunächst herausgestellt werden. Abschließend wird geprüft, ob es sich bei der Emotionalen Intelligenz um eine vorwiegend weibliche Kompetenz handelt
Automated evaluation of contact angles in a three-phase system of selective agglomeration in liquids
(2020)
This study aims to an automated evaluation of contact angles in a three-phase system of selective agglomeration in liquids. Wetting properties, quantified by contact angles, are essential in many industries and their processes. Selective agglomeration as a three-phase system consists of a suspension liquid, a heterogeneous solid phase and an immiscible binding liquid. It offers the chance of establishing more efficient separation processes because of the shape-dependent wetting properties of fine particles (size ≤ 10 µm). In the present paper, an experimental setup for contact angle measurements of fine particles based on the Sessile Drop Method is described. Moreover, a new algorithm is discussed, which can be used to automatically compute contact angles from image data captured by a high-speed camera. The algorithm uses a marker-based watershed transform to segment the image data into regions representing the droplet, the carrier plate coated by fine particles, and the background. The main idea is a parametric modelling approach forthe time-dependent droplet’s contour by an ellipse.
The results show that the development of the dynamic contact angles towards a static contact angle can be efficiently determined based on this novel technique. These findings are useful for a detailed discrimination of wetting properties of spherical and irregularly shaped particles as well as their wetting kinetics. Also, a better understanding of selective agglomeration processes will be promoted by this user-friendly method.
Implementation strategies of a modern showroom concept for retailers with a wide range of products
(2022)
This paper suggests a new business model based on modern technology for retail. In the age of digitalization, stationary retail is losing market shares to online retail. Therefore, there is an obvious need for change in businesses. The concept developed in this paper combines the strengths of online and stationary retail to benefit stationary retail. In the approach taken in this paper, the basis for change is modern technology. Finding innovative ways to use technologies like NFC, AI, and robotics is regarded as the key factor to sustainable success. As the implementation of modern technologies entails a particular investment, the customers’ opinion on structural changes like these has been included in the consideration. Therefore, a survey has been conducted to find out which level of innovation current customers are willing to accept thus emphasizing the need for certain changes and dissuading specific others. The result of this paper is the modern showroom concept which takes the customers’ opinion into account while implementing the right amount of technology that should pave the way to a sustainable future for stationary retail.
Radar signal processing is a promising tool for vital sign monitoring. For contactless observation of breathing and heart rate a precise measurement of the distance between radar antenna and the patient’s skin is required. This results in the need to detect small movements in the range of 0.5 mm and below. Such small changes in distance are hard to be measured with a limited radar bandwidth when relying on the frequency based range detection alone. In order to enhance the relative distance resolution a precise measurement of the observed signal’s phase is required. Due to radar reflections from surfaces in close proximity to the main area of interest the desired signal of the radar reflection can get superposed. For superposing signals with little separation in frequency domain the main lobes of their discrete Fourier transform (DFT) merge into a single lobe, so that their peaks cannot be differentiated. This paper evaluates a method for reconstructing the phase and amplitude of such superimposed signals.
Productive biofilms are gaining growing interest in research due to their potential of producing valuable compounds and bioactive substances such as antibiotics. This is supported by recent developments in biofilm photobioreactors that established the controlled phototrophic cultivation of algae and cyanobacteria. Cultivation of biofilms can be challenging due to the need of surfaces for biofilm adhesion. The total production of biomass, and thus production of e.g. bioactive substances, within the bioreactor volume highly depends on the available cultivation surface. To achieve an enlargement of surface area for biofilm photobioreactors, biocarriers can be implemented in the cultivation. Thereby, material properties and design of the biocarriers are important for initial biofilm formation and growth of cyanobacteria. In this study, special biocarriers were designed and additively manufactured to investigate different polymeric materials and surface designs regarding biofilm adhesion of the terrestrial cyanobacterium Nostoc flagelliforme (CCAP 1453/33). Properties of 3D-printed materials were characterized by determination of wettability, surface roughness, and density. To evaluate the influence of wettability on biofilm formation, material properties were specifically modified by gas-phase fluorination and biofilm formation was analyzed on biocarriers with basic and optimized geometry in shaking flask cultivation. We found that different polymeric materials revealed no significant differences in wettability and with identical surface design no significant effect on biomass adhesion was observed. However, materials treated with fluorination as well as optimized biocarrier design showed improved wettability and an increase in biomass adhesion per biocarrier surface.
As productive biofilms are increasingly gaining interest in research, the quantitative monitoring of biofilm formation on- or offline for the process remains a challenge. Optical coherence tomography (OCT) is a fast and often used method for scanning biofilms, but it has difficulty scanning through more dense optical materials. X-ray microtomography (μCT) can measure biofilms in most geometries but is very time-consuming. By combining both methods for the first time, the weaknesses of both methods could be compensated. The phototrophic cyanobacterium Tolypothrix distorta was cultured in a moving bed photobioreactor inside a biocarrier with a semi-enclosed geometry. An automated workflow was developed to process µCT scans of the biocarriers. This allowed quantification of biomass volume and biofilm-coverage on the biocarrier, both globally and spatially resolved. At the beginning of the cultivation, a growth limitation was detected in the outer region of the carrier, presumably due to shear stress. In the later phase, light limitations could be found inside the biocarrier. µCT data and biofilm thicknesses measured by OCT displayed good correlation. The latter could therefore be used to rapidly measure the biofilm formation in a process. The methods presented here can help gain a deeper understanding of biofilms inside a process and detect any limitations.