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Techniques like speckle holography and shearography are rarely applied due to the complexity of instrument setup and lack of automated result analysis, despite their potential. By simulating speckle interferometric outcomes, we seek to address these challenges, enabling more efficient measurement processes and paving the way for automated defect recognition. This research focuses on developing a simulation code for speckle interferometric results derived from finite element analyses. The aim is to improve the parameter settings of speckle interferometry measurements and create specific datasets, which will be used to develop machine learning-based methods for automation in series production.
Cellular adhesion and contractility are essential for cell movement. In this study, we investigated the effects of actin stabilization on adhesion properties, contractility, and cell migration. For this, we used the recently synthesized actin stabilizer miuraenamide A (MiuA), which has been discussed as a more reliable alternative to the otherwise commonly used actin stabilizer jasplakinolide. We investigated the number and size of focal adhesions in RPE-1 cells and used single-cell force spectroscopy to evaluate the adhesion properties of those cells after MiuA treatment. We showed that MiuA increases the number of focal adhesions while decreasing their size and reduces adhesion energy and force. Additionally, we investigated its effects on the contractility of RPE-1 cells by measuring their contractile energy using pattern-based contractility screening (PaCS). We found no significant change in contractility after MiuA treatment. Finally, we confined RPE-1 cells in PDMS microchannels and analyzed their migration after treatment with MiuA, showing that neither their speed nor their persistence is affected by MiuA. To check that these effects are not specific to RPE-1 cells, we also analyzed the effects of MiuA treatment in MEF cells and neutrophils. Both MEF cells and neutrophils showed the same results as the RPE-1 cells. Our measurements indicate that, although altering focal adhesions significantly reduces adhesion, it does not impact cell contractility. This finding also clarifies why amoeboid migration, which operates independently of adhesion, remains unaffected. Additionally, it explains the previously observed reduction in mesenchymal migration, which relies on adhesion-based mechanisms.
This study investigates the effects of mechanical strain on the surface roughness of copper conductors, focusing on the electrolyte-refined copper (Cu-ETP, CW004A) used in H07V-U 1.5 mm2 single-core cables. For the first time, the surface roughness evolution is characterized using the power spectral density (PSD) function, enabling a detailed roughness analysis across different spatial length scales. Conductors were subjected to mechanical stress, with measurements taken at multiple stages of service life. The study confirms the results from other studies that surface roughness increases significantly in the early stages of loading, with a plateau observed in 50 % - 75 % of cycles to failure. Micro crack formation and material extrusion are identified as key mechanisms driving roughness growth, especially at small length scales, with a shift towards larger length scales as strain intensifies. The increasing Hurst exponent suggests a transformation from a random to a more persistent and correlated surface. The results underscore the potential of power spectral density analysis in understanding surface behavior in copper conductors.
Relative movements between electrodes and tissue are a potential source of interference signals that can affect the accuracy of signal recordings and data analyses. To address this challenge, a test bench is developed that utilises mechanical excitation to impose dynamic flow on the electrodes under various conditions to characterize the influence on signal quality. The generated data can then be recorded and analysed with the Fast Fourier Transformation.
Staphylococcus aureus (S. aureus) is one of the bacterial species capable of forming multilayered biofilms on implants. Such biofilms formed on implanted medical devices often require the removal of the implant in order to avoid sepsis or, in the worst case, even the death of the patient. To address the problem of unwanted S. aureus biofilm formation, its first step, i.e., adhesion, must be understood and prevented. Thus, the development of adhesion-reducing surface coatings for implant materials is of utmost importance. In this work, we used single-cell force spectroscopy to analyze the adhesion of the biofilm-forming S. aureus strain SA113 on naive and protein-coated silicon surfaces (SiO2). In addition to the wild type, we used the SA113 ΔdltA knockout mutant to further investigate the effect of d-alanylation of lipoteichoic acids of the cell wall. In order to examine how the surface charge affects adhesion, we coated silanized SiO2 surfaces with amphiphilic class II hydrophobins. The naturally occurring hydrophobin HFBI was used as well as the HFBI variant D40Q/D43N, which is less negatively charged at physiological pH due to the exchange of two acidic aspartate residues. These two types of hydrophobin-coated surfaces resemble each other in roughness and wettability but differ only in charge. By measurement of the forces with which each S. aureus strain binds to hydrophobin-coated surfaces, we show that the adhesion of S. aureus at surfaces can be influenced by the charges exposed by the target surfaces. Therefore, in addition to hydrogen bonding, electrostatic interactions between the cell and the hydrophilic surface govern adhesion on these surfaces. Moreover, we found that for both HFBI coatings, the adhesion strength of S. aureus is reduced by nearly a factor of 30 compared to silanized SiO2 surfaces. Therefore, hydrophobin coatings are of great interest for further use in the field of biomedical surface coating.
Mechanoelectrical Effects in Natural Fiber-Reinforced Polymers as Structural Health Monitoring
(2025)
Natural fiber-reinforced polymers are gaining popularity as sustainable structural materials. However, their inherent variability can limit their reliability in load-bearing applications. To address this issue, we investigate a novel structural health monitoring method that leverages mechanoelectrical effects in flax fiber-reinforced epoxy composites. In our study, a contactless capacitive coupled measurement setup records electrical polarization during fatigue testing at four load levels. The polarization signals we observed increased with increasing load levels. Additionally, changes in polarization correlate with changes in dynamic modulus, providing early indicators of potential failure. This work lays the foundation for a new type of structural health monitoring in natural fiber-reinforced polymers.
This work explores the combination of direct numerical simulations (DNSs) and experimental approaches for studying technical emulsification processes. Although emulsions have long been used in a variety of industries and many important research papers have been published over the years, quantifying and predicting the dispersion of droplets in another liquid remains challenging because of the complex multiphase nature and microscopic droplet scales. This study focuses on water-in-gasoline emulsions, which have the potential to improve efficiency and reduce emissions in combustion-based power generation. Experimental data from two different emulsion injection systems are complemented with DNS to gain insight into emulsification and the resulting droplet size distribution. In situ shadow imaging is used to acquire the experimental droplet size distributions, whereas DNS is performed via the geometric volume of fluid (VoF) method with the open-source code PARIS. The results indicate consistent agreement between the experimental and simulation results. Additionally, a corresponding trend of increasing droplet size is observed as the volume fraction of the dispersed phase increases. Furthermore, a detailed analysis of various probability density functions for modeling droplet size distributions (DSDs) reveals that the gamma distribution is the most appropriate. Overall, this work demonstrates that DNS can be successfully combined with experiments to increase the understanding of emulsification processes.
Impact of Geometry on Chemical Analysis Exemplified for Photoelectron Spectroscopy of Black Silicon
(2025)
For smooth surfaces, chemical composition can be readily analyzed using various spectroscopic techniques, a prominent example is X-ray photoelectron spectroscopy (XPS), where the relative proportions of the elements are mainly determined by the intensity ratio of the element-specific photoelectrons. However, this analysis becomes more complex for nanorough surfaces like black silicon (b-Si) due to the geometry's steep slopes, which mimic local variations in emission angles. In this study, this effect is explicitly quantified through an integral geometric analysis using Minkowski tensors, correlating XPS chemical data with topographical information from Atomic Force Microscopy (AFM). This approach yields reliable estimates of layer thicknesses for nanorough surfaces. For b-Si, it is found that the oxide layer is ≈50%–80% thicker than the native oxide layer on a standard Si wafer. This study underscores the significant impact of nanoscale geometries on chemical property analysis.
The Active Radar Interferometer (AcRaIn) represents a novel approach in secondary radar technology, aimed at environments with high reflective clutter, such as pipes and tunnels. This study introduces a compact design minimizing peripheral components and leveraging commercial semiconductor technologies operating in the 24 GHz ISM band. A heterodyne principle was adopted to enhance unambiguity and phase coherence without requiring synchronization or separate communication channels. Experimental validation involved free-space and pipe measurements, demonstrating functionality over distances up to 150 m. The radar system effectively reduced interference and achieved high precision in both straight and bent pipe scenarios, with deviations below 1.25% compared to manual measurements. By processing signals at intermediate frequencies, advantages such as improved efficiency, isolation, and system flexibility were achieved. Notably, the integration of amplitude modulation suppressed passive clutter, enabling clearer signal differentiation. Key challenges identified include optimizing signal processing and addressing logarithmic signal attenuation for better precision. These findings underscore AcRaIn’s potential for pipeline monitoring and similar applications.
(1) Objective: This study aims to lay a foundation for noncontact intensive care monitoring of premature babies.
(2) Methods: Arterial oxygen saturation and heart rate were measured using a monochrome camera and time-division multiplex controlled lighting at three different wavelengths (660 nm, 810 nm and 940 nm) on a piglet model.
(3) Results: Using this camera system and our newly designed algorithm for further analysis, the detection of a heartbeat and the calculation of oxygen saturation were evaluated. In motionless individuals, heartbeat and respiration were separated clearly during light breathing and with only minor intervention. In this case, the mean difference between noncontact and contact saturation measurements was 0.7% (RMSE = 3.8%, MAE = 2.93%).
(4) Conclusions: The new sensor was proven effective under ideal animal experimental conditions. The results allow a systematic improvement for the further development of contactless vital sign monitoring systems. The results presented here are a major step towards the development of an incubator with noncontact sensor systems for use in the neonatal intensive care unit.