Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification

  • Purification of mRNA with oligo(dT)-functionalized magnetic particles involves a series of magnetic separations for buffer exchange and washing. Magnetic particles interact and agglomerate with each other when a magnetic field is applied, which can result in a decreased total surface area and thus a decreased yield of mRNA. In addition, agglomeration may also be caused by mRNA loading on the magnetic particles. Therefore, it is of interest how the individual steps of magnetic separation and subsequent redispersion in the buffers used affect the particle size distribution. The lysis/binding buffer is the most important buffer for the separation of mRNA from the multicomponent suspension of cell lysate. Therefore, monodisperse magnetic particles loaded with mRNA were dispersed in the lysis/binding buffer and in the reference system deionized water, and the particle size distributions were measured. A concentration-dependent agglomeration tendency was observed in deionized water. In contrast, no significant agglomeration was detected in the lysis/binding buffer. With regard to magnetic particle recycling, the influence of different storage and drying processes on particle size distribution was investigated. Agglomeration occurred in all process alternatives. For de-agglomeration, ultrasonic treatment was examined. It represents a suitable method for reproducible restoration of the original particle size distribution.

Volltext Dateien herunterladen

Metadaten exportieren

Metadaten
Verfasserangaben:Lars Wommer, Winda Soerjawinata, Roland Ulber, Percy Kampeis
URN:urn:nbn:de:hbz:tr5-1107
DOI:https://doi.org/10.1002/elsc.202000112
Titel des übergeordneten Werkes (Englisch):Engineering in Life Sciences
Verlag:Wiley
Dokumentart:Wissenschaftlicher Artikel (Fachzeitschriften)
Sprache:Englisch
Datum des OPUS-Uploads:03.09.2022
Datum der Erstveröffentlichung:24.06.2021
Veröffentlichende Hochschule:Hochschule Trier
Datum der Freischaltung:05.09.2022
Freies Schlagwort / Tag:agglomeration; de-agglomeration; high-gradient magnetic separation; mRNA-vaccines; magnetic beads
GND-Schlagwort:Magnetisches Trennverfahren; mRNA-Impfstoff; Agglomerieren
Jahrgang:21
Ausgabe / Heft:10
Seitenzahl:15
Erste Seite:558
Letzte Seite:572
Einrichtungen:FB Umweltplanung/-technik (UCB)
DDC-Klassifikation:6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik
Lizenz (Deutsch):License LogoCreative Commons - CC BY - Namensnennung 4.0 International