TY  - JOUR
A1  - Soerjawinata, Winda
A1  - Prajapati, Shila
A1  - Barth, Isabelle
A1  - Lu, Xiaohua
A1  - Ulber, Roland
A1  - Efferth, Thomas
A1  - Kampeis, Percy
T1  - Production of Protease Inhibitor With Penicillium sp. — Optimization of the Medium for Growth in Pellet Form and Cytotoxicity Testing
T2  - Engineering in Life Sciences
N2  - Penicillium sp. (IBWF 040-09) produces a protease inhibitor that can potentially be used against the main protease of human African trypanosomiasis. Since the target substance is formed intracellularly (under nutrient limitation), the fungal pellet is preferred compared to the free mycelia in bioreactor cultivation. The optimization of the production of protease inhibitor became the main focus of this study. The effects of the concentrations of spores, calcium chloride, and Pluronic F68 were investigated with regard to fungal growth, pellet morphology, and the production of protease inhibitor. The combination of adjusting the spore concentration and adding Pluronic F68 and calcium chloride increased the probability of achieving the desired morphology. This ensured better reproducibility of the production of the target substance by Penicillium sp. (IBWF 040-09) with the bioreactor system used. In addition, the protease inhibitor was tested in a resazurin assay and showed no noticeable cytotoxic effects on peripheral blood mononuclear cells isolated from whole blood cells.
KW  - cytotoxicity testing
KW  - fungal cultivation
KW  - fungal growth
KW  - pellet morphology
KW  - protease inhibitor
KW  - Penicillium
KW  - Trypanosomiase
KW  - Proteaseinhibitor
KW  - Produktion
KW  - Bioreaktor
KW  - Pluronic
KW  - Calciumchlorid
KW  - Cytotoxizität
Y1  - 2025
UR  - https://hst.opus.hbz-nrw.de/frontdoor/index/index/docId/1046
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:tr5-10467
VL  - 25
IS  - 3
SP  - 1
EP  - 10
PB  - Wiley
ER  -