TY  - JOUR
A1  - Soerjawinata, Winda
A1  - Kockler, Isabelle
A1  - Wommer, Lars
A1  - Frank, Robert
A1  - Schüffler, Anja
A1  - Schirmeister, Tanja
A1  - Ulber, Roland
A1  - Kampeis, Percy
T1  - Novel bioreactor internals for the cultivation of spore-forming fungi in pellet form
T2  - Engineering in Life Sciences
N2  - This study introduced an automated long-term fermentation process for fungals grown in pellet form. The goal was to reduce the overgrowth of bioreactor internals and sensors while better rheological properties in the fermentation broth, such as oxygen transfer and mixing time, can be achieved. Because this could not be accomplished with continuous culture and fed-batch fermentation, repeated-batch fermentation was implemented with the help of additional bioreactor internals (“sporulation supports”). This should capture some biomass during fermentation. After harvesting the suspended biomass, intermediate cleaning was performed using a cleaning device. The biomass retained on the sporulation support went through the sporulation phase. The spores were subsequently used as inocula for the next batch. The reason for this approach was that the retained pellets could otherwise cause problems (e.g., overgrowth on sensors) in subsequent batches because the fungus would then show undesirable hyphal growth. Various sporulation supports were tested for sufficient biomass fixation to start the next batch. A reproducible spore concentration within the range of the requirements could be achieved by adjusting the sporulation support (design and construction material), and an intermediate cleaning adapted to this.
KW  - Bioreaktor
KW  - Fermentation
KW  - Sporenbildung
KW  - bioreactor internals
KW  - fungal growth
KW  - fungal pellets
KW  - repeated-batch fermentation
KW  - sporulation conditions
Y1  - 2022
UR  - https://hst.opus.hbz-nrw.de/frontdoor/index/index/docId/127
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:tr5-1270
VL  - 22
IS  - 7
SP  - 474
EP  - 483
PB  - Wiley
ER  -