TY  - JOUR
A1  - Soerjawinata, Winda
A1  - Schlegel, Konstantin
A1  - Fuchs, Natalie
A1  - Schüffler, Anja
A1  - Schirmeister, Tanja
A1  - Ulber, Roland
A1  - Kampeis, Percy
T1  - Applicability of a single-use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form
T2  - Engineering in Life Sciences
N2  - The implementation of single-use technologies offers several major advantages, e.g. prevention of cross-contamination, especially when spore-forming microorganisms are present. This study investigated the application of a single-use bioreactor in batch fermentation of filamentous fungus Penicillium sp. (IBWF 040-09) from the Institute of Biotechnology and Drug Research (IBWF), which is capable of intracellular production of a protease inhibitor against parasitic proteases as a secondary metabolite. Several modifications to the SU bioreactor were suggested in this study to allow the fermentation in which the fungus forms pellets. Simultaneously, fermentations in conventional glass bioreactor were also conducted as reference. Although there are significant differences in the construction material and gassing system, the similarity of the two types of bioreactors in terms of fungal metabolic activity and the reproducibility of fermentations could be demonstrated using statistic methods. Under the selected cultivation conditions, growth rate, yield coefficient, substrate uptake rate, and formation of intracellular protease-inhibiting substance in the single-use bioreactor were similar to those in the glass bioreactor.
KW  - Bioreaktor
KW  - Fermentation
KW  - Pellet
KW  - Proteaseinhibitor
KW  - Penicillium
KW  - Penicillium sp.
KW  - fermentation
KW  - pellets
KW  - protease inhibitor
KW  - single‐use bioreactor
Y1  - 2021
UR  - https://hst.opus.hbz-nrw.de/frontdoor/index/index/docId/111
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:tr5-1113
VL  - 21
IS  - 5
SP  - 324
EP  - 339
PB  - Wiley
ER  -