TY  - JOUR
A1  - Scherer, Kai
A1  - Soerjawinata, Winda
A1  - Schaefer, Susanne
A1  - Kockler, Isabelle
A1  - Ulber, Roland
A1  - Lakatos, Michael
A1  - Bröckel, Ulrich
A1  - Kampeis, Percy
A1  - Wahl, Michael
T1  - Influence of wettability and surface design on the adhesion of terrestrial cyanobacteria to additive manufactured biocarriers
T2  - Bioprocess and Biosystems Engineering
N2  - Productive biofilms are gaining growing interest in research due to their potential of producing valuable compounds and bioactive substances such as antibiotics. This is supported by recent developments in biofilm photobioreactors that established the controlled phototrophic cultivation of algae and cyanobacteria. Cultivation of biofilms can be challenging due to the need of surfaces for biofilm adhesion. The total production of biomass, and thus production of e.g. bioactive substances, within the bioreactor volume highly depends on the available cultivation surface. To achieve an enlargement of surface area for biofilm photobioreactors, biocarriers can be implemented in the cultivation. Thereby, material properties and design of the biocarriers are important for initial biofilm formation and growth of cyanobacteria. In this study, special biocarriers were designed and additively manufactured to investigate different polymeric materials and surface designs regarding biofilm adhesion of the terrestrial cyanobacterium Nostoc flagelliforme (CCAP 1453/33). Properties of 3D-printed materials were characterized by determination of wettability, surface roughness, and density. To evaluate the influence of wettability on biofilm formation, material properties were specifically modified by gas-phase fluorination and biofilm formation was analyzed on biocarriers with basic and optimized geometry in shaking flask cultivation. We found that different polymeric materials revealed no significant differences in wettability and with identical surface design no significant effect on biomass adhesion was observed. However, materials treated with fluorination as well as optimized biocarrier design showed improved wettability and an increase in biomass adhesion per biocarrier surface.
KW  - Rapid Prototyping <Fertigung>
KW  - Biofilm
KW  - Photoreaktor
KW  - Cyanobakterien
KW  - additive manufacturing
KW  - biocarriers
KW  - biofilm
KW  - photobioreactor
KW  - terrestrial cyanobacteria
Y1  - 2022
UR  - https://hst.opus.hbz-nrw.de/frontdoor/index/index/docId/108
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:tr5-1084
VL  - 45
IS  - 5
SP  - 931
EP  - 941
PB  - Springer Nature
ER  -