TY  - JOUR
A1  - Stiefelmaier, Judith
A1  - Strieth, Dorina
A1  - Schaefer, Susanne
A1  - Wrabl, Björn
A1  - Kronenberger, Daniel
A1  - Bröckel, Ulrich
A1  - Ulber, Roland
T1  - A new easy method for determination of surface adhesion of phototrophic biofilms
T2  - Biotechnology and Bioengineering
N2  - Terrestrial cyanobacteria grow as phototrophic biofilms and offer a wide spectrum of interesting products. For cultivation of phototrophic biofilms different reactor concepts have been developed in the last years. One of the main influencing factors is the surface material and the adhesion strength of the chosen production strain. In this work a flow chamber was developed, in which, in combination with optical coherence tomography and computational fluid dynamics simulation, an easy analysis of adhesion forces between different biofilms and varied surface materials is possible. Hereby, differences between two cyanobacteria strains and two surface materials were shown. With longer cultivation time of biofilms adhesion increased in all experiments. Additionally, the content of extracellular polymeric substances was analyzed and its role in surface adhesion was evaluated. To test the comparability of obtained results from the flow chamber with other methods, analogous experiments were conducted with a rotational rheometer, which proved to be successful. Thus, with the presented flow chamber an easy to implement method for analysis of biofilm adhesion was developed, which can be used in future research for determination of suitable combinations of microorganisms with cultivation surfaces on lab scale in advance of larger processes.
KW  - adhesion
KW  - flow chamber
KW  - optical coherence tomography
KW  - phototrophic biofilms
KW  - rotational rheometer
KW  - Adhäsion
KW  - Biofilm
KW  - Cyanobakterien
KW  - Viskosimeter
KW  - Optische Kohärenztomografie
Y1  - 2023
UR  - https://hst.opus.hbz-nrw.de/frontdoor/index/index/docId/1007
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:tr5-10077
VL  - 120
IS  - 12
SP  - 3518
EP  - 3528
PB  - John Wiley & Sons
ER  -